biotinylated pna Search Results


peanut agglutinin pna  (Vector Laboratories)
95
Vector Laboratories peanut agglutinin pna
Peanut Agglutinin Pna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/Biotinylated+Peanut+Agglutinin+(PNA)/10__1002_slash_j__1939___4640__2003__tb02640__x-66-19-50
Average 95 stars, based on 1 article reviews
peanut agglutinin pna - by Bioz Stars, 2026-09
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biotinylated lotus tetragonolobus agglutinin  (Vector Laboratories)
93
Vector Laboratories biotinylated lotus tetragonolobus agglutinin
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Lotus Tetragonolobus Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/Biotinylated+Anti-Peanut+Agglutinin+(PNA)/pm26236989-496-13-19
Average 93 stars, based on 1 article reviews
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biotinylated peanut agglutinin or pna ba-2301-1 antibody  (EY Laboratories)
90
EY Laboratories biotinylated peanut agglutinin or pna ba-2301-1 antibody
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Peanut Agglutinin Or Pna Ba 2301 1 Antibody, supplied by EY Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/biotinylated+peanut+agglutinin+or+pna+ba+2301+1+antibody/pmc04646690-111-17-21
Average 90 stars, based on 1 article reviews
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5′-biotinyl pna-sl3-17  (PANAGENE Inc)
90
PANAGENE Inc 5′-biotinyl pna-sl3-17
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
5′ Biotinyl Pna Sl3 17, supplied by PANAGENE Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/5++biotinyl+pna+sl3+17/pmc05913291-273-9-15
Average 90 stars, based on 1 article reviews
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biotinylated pna  (J-Oil Mills Inc)
90
J-Oil Mills Inc biotinylated pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Pna, supplied by J-Oil Mills Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/biotinylated+pna/pm35915223-263-24-27
Average 90 stars, based on 1 article reviews
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arachis hypogaea (peanut) agglutinin (pna) fluorescein isothiocyanate (fitc)  (Seikagaku corporation)
90
Seikagaku corporation arachis hypogaea (peanut) agglutinin (pna) fluorescein isothiocyanate (fitc)
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Arachis Hypogaea (Peanut) Agglutinin (Pna) Fluorescein Isothiocyanate (Fitc), supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/peanut+agglutinin++pna++biotinylated+arachis+hypogaea+++lectin/pmc05907117-89-8-16
Average 90 stars, based on 1 article reviews
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biotinylated hplc-purified peptide nucleic acid (pna) probes  (Metabion International AG)
90
Metabion International AG biotinylated hplc-purified peptide nucleic acid (pna) probes
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Hplc Purified Peptide Nucleic Acid (Pna) Probes, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/biotinylated+hplc+purified+peptide+nucleic+acid++pna++probes/pm11570491-36-3-11
Average 90 stars, based on 1 article reviews
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biotinylated pna  (GALAB Technologies GmbH)
90
GALAB Technologies GmbH biotinylated pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Pna, supplied by GALAB Technologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/biotinylated+pna+antibody/pmc05391454-385-19-23
Average 90 stars, based on 1 article reviews
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biotin-conjugated pna  (Seikagaku corporation)
90
Seikagaku corporation biotin-conjugated pna
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotin Conjugated Pna, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/biotinylated+pna/pmc09223859-197-17-21
Average 90 stars, based on 1 article reviews
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biotinylated pna antibody  (Becton Dickinson)
90
Becton Dickinson biotinylated pna antibody
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Pna Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+pna/biotinylated+pna+antibody/pm24745335-222-23-28
Average 90 stars, based on 1 article reviews
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biotinylated γpna reporter probes  (PNA Innovations)
90
PNA Innovations biotinylated γpna reporter probes
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated γpna Reporter Probes, supplied by PNA Innovations, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated telomere-specific pna capture probes pctel-bio  (PCTel Inc)
90
PCTel Inc biotinylated telomere-specific pna capture probes pctel-bio
Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus <t>tetragonolobus</t> <t>agglutinin)</t> + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.
Biotinylated Telomere Specific Pna Capture Probes Pctel Bio, supplied by PCTel Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus tetragonolobus agglutinin) + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.

Journal: Nature medicine

Article Title: Snail1-induced partial epithelial-to-mesenchymal transition drives renal fibrosis in mice and can be targeted to reverse established disease.

doi: 10.1038/nm.3901

Figure Lengend Snippet: Figure 2 Renal epithelial cells undergo a partial EMT after UUO. (a) Ksp1.3-Cre;tdTomatofl/fl mice were subjected to UUO and kidneys were collected 15 ds after surgery. Representative images (n = 20) of sections of kidneys from Ksp1.3-Cre;tdTomatofl/fl mice (KCT) 15 d after being subjected to UUO. Images are for obstructed (KCT + UUO) and contralateral non-obstructed kidneys (Control) from KCT mice stained for LTA (Lotus tetragonolobus agglutinin) + PNA (peanut agglutinin) (markers of proximal and distal tubules17), α-SMA and DAPI. tdTomato fluorescent protein expression was directly visualized. Asterisks (*) indicate α-SMA expression in tubular cells that have lost lectin expression 15 d after UUO. dt, dilated tubule; g, glomerulus; t, tubule. (b) Number of dilated and total tubules in kidneys from WT and SFKC mice 15 d after UUO. Ten different fields were counted in 12 mice per group. Data represent mean ± s.e.m. *P < 0.05, NS, nonsignificant; Mann-Whitney test. (c) Representative images (n = 5) of double Snail1 and α-SMA immunolabeling in sections from obstructed kidneys of WT mice euthanized 15 d after surgery. White asterisks indicate Snail1-positive nuclei. Green asterisks indicate double-positive Snail1 and α-SMA tubular cells. (d) Representative images (n = 5) of lectin (LTA) and Snail1 immunofluorescence in sections from the same kidneys used in b. Scale bars, 50 µm.

Article Snippet: The sections were incubated overnight at 4 °C with the following primary antibodies: biotinylated Lotus tetragonolobus agglutinin, LTA (B-1325, Vector Laboratories, Burlingame, CA); biotinylated peanut agglutinin, PNA (B-1075, Vector Laboratories, Burlingame, CA); α smooth muscle actin, (α-SMA) (Sigma-Aldrich, St Louis, MO); vimentin (sc-7557, Santa Cruz Biotechnology, Santa Cruz, CA); Snail1 (Ab1773; Abcam PLC, Cambridge, MA); phosphoNF-κB p65 (Ser276) (3037S, Cell Signaling Technology, Inc., Danvers, MA); phospho-Smad2 (Ser465/467) (3101S, Cell Signaling Technology, Inc., Danvers, MA); F4/80 (MCA497GA, Serotec, Oxford,UK); estrogen receptor alpha, ER-α (sc-543, Santa Cruz Biotechnology, Santa Cruz, CA); CD163 (sc-18796 Santa Cruz Biotechnology, Santa Cruz, CA).

Techniques: Control, Staining, Expressing, MANN-WHITNEY, Immunolabeling, Immunofluorescence